Characterization of Nora Virus Structural Proteins via Western Blot Analysis

Characterization of Nora Virus Structural Proteins via Western Blot Analysis

Nora virus is a single stranded RNA picorna-like virus with four open reading frames (ORFs). The coding potentials of the ORFs are not fully characterized, but ORF3 and ORF4 are believed to encode the capsid proteins (VP3, VP4a, VP4b, and VP4c) comprising the virion. To determine the polypeptide composition of Nora virus virions, polypeptides from purified virus were compared to polypeptides de...

Characterization of Aphanomyces invadans by electrophoretic and Western blot analysis

Fungal mycelium extracts were subjected to gel electrophoresis and Western blot analysis with various lectins and polyclonal antisera. These techniques succeeded in identifying bands specific to pathogenic Aphanomyces strains from fish affected by epizootic ulcerative syndrome, redspot disease and mycotic granulomatosis when compared to various other Oomycete fungi. It is concluded that these i...

Genetic Analysis of Three Structural Proteins in Iranian Infectious Bronchitis Virus Isolate

Infectious bronchitis virus (IBV) is a contagious pathogen in fowl that results in economic loss in the poultry industry. In this study, the amino acids sequences of three structural proteins M, N, and S1 for five Iranian IBV isolated during 1998-2011 have been analyzed. Conserved and variable regions, hydrophobic characteristics and identity matrix were determined after alignment by Bioedit ve...

Western blot of stained proteins from dried polyacrylamide gels.

Western blot analysis in patients with hypocaeruloplasminaemia.

Inherited copper toxic disease, Wilson's disease, is an autosomal recessive disorder arising from a defect in biliary copper excretion. Although there are several pathognomonic clinical features, such a multisystem disease can be difficult to diagnose, particularly in the early stages of copper toxicity. Even measurements of serum copper and caeruloplasmin, the major copper-transporting protein...